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. 2021 May 13;218(4):iyab073. doi: 10.1093/genetics/iyab073

Figure 6.

Figure 6

3-O-sulfation of SDN-1 is required for stabilization of MADD-4 at NMJs. (A) Confocal images of MADD-4-tagRFPt knock-in (magenta) and mNG-SDN-1 knock-in (green) at the DNC of sdn-1(+/-) heterozygous worms. Quantifications show the MADD-4 (left) and SDN-1 (right) fluorescence intensity at DNC in each genotype and corresponding results of Mann–Whitney test. (B) Confocal images of MADD-4-tagRFPt knock-in (magenta) and mNG-SDN-1 knock-in (green) at the DNC of madd-4(+/-) heterozygous worms. Quantifications show the MADD-4 (left) and SDN-1 (right) fluorescence intensity at DNC in each genotype and corresponding results of Mann–Whitney test. (C, D) Confocal images of MADD-4-tagRFPt knock-in at the DNC (C) and VNC (D) of control, hst-3.1(tm734); hst-3.2(tm3006), sdn-1(zh20), and hst-3.1(tm734); hst-3.2(tm3006) sdn-1(zh20) worms. Quantifications show the MADD-4 fluorescence intensity at DNC (C) and VNC (D) in each genotype and corresponding results of Dunn’s multiple comparison test. Scale bar, 5 µm.