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Removing 6-O-sulfation rescues MADD-4 levels in the absence of 3-O-sulfation. (A–D) Confocal images of MADD-4-tagRFPt knock-in (magenta) at the DNC of control (A) and triple mutants: hst-3.1(tm734); hse-5(tm472); hst-3.2(tm3006) (B), hst-3.1(tm734); hst-3.2(tm3006) hst-2 (ok595) (C), hst-3.1(tm734); hst-3.2(tm3006) hst-6(ok273) (D). (E) Quantifications show the MADD-4 fluorescence intensity at DNC in each genotype and results of Dunn’s multiple comparison test. Scale bar, 5 µm.
