Table 1.
hse-5, hst-2, hst-6, and sul-1 are individually dispensable for NMJ organization
| MADD-4 |
L-AChRs (UNC-29) |
GABARs (UNC-49) |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Controls |
Mutants |
P-value | Controls |
Mutants |
P-value | Controls |
Mutants |
P-value | |||||||
| Mean ± SEM (%) | N | Mean ± SEM (%) | N | Mean ± SEM (%) | N | Mean ± SEM (%) | N | Mean ± SEM (%) | N | Mean ± SEM (%) | N | ||||
| hse-5(tm472) | 100 ± 7 | 25 | 100 ± 7 | 25 | 0.99 | 100 ± 4 | 23 | 108 ± 4 | 25 | 0.08 | 100 ± 7 | 32 | 81 ± 3 | 31 | 0.06 |
| hst-2(ok595) | 100 ± 11 | 18 | 124 ± 10 | 18 | 0.08 | 100 ± 6 | 24 | 99 ± 6 | 16 | 0.94 | 100 ± 19ª | 24 | 110 ± 19ª | 26 | 0.46 |
| hst-6(ok273) | 100 ± 5 | 26 | 107 ± 4 | 36 | 0.26 | 100 ± 5 | 30 | 90 ± 6 | 28 | 0.11 | 100 ± 4 | 34 | 93 ± 4 | 29 | 0.32 |
| sul-1(gk151) | 100 ± 6 | 24 | 110 ± 5 | 21 | 0.28 | 100 ± 5 | 26 | 101 ± 4 | 21 | 0.81 | 100 ± 4 | 20 | 114 ± 5 | 22 | 0.01 |
Quantification of the fluorescence intensity of MADD-4 (MADD-4-tagRFPt knock-in), L-AChRs (UNC-29-tagRFPt knock-in), and GABARs (UNC-49-tagRFPt knock-in), at the DNC, in hse-5(tm472), hst-2(ok595), hst-6(ok273), and sul-1(gk151) mutants, except for hst-2(ok595), where GABARs were quantified in UNC-49-pHluorin knock-in (a). Quantifications are expressed as a percentage of mean control intensity ± standard error of the mean (SEM) and P-values are calculated using the Mann–Whitney test.