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Roles of Cup9-regulated genes in cell wall integrity. Comparisons of deletion mutants were conducted. The wild-type strain (CW542), orf19.3499Δ/Δ orf19.851Δ/Δ pga28Δ/Δ triple mutant (CW1697), orf19.851Δ/Δ mutant (YI459), orf19.3499Δ/Δ mutant (YI463), pga28Δ/Δ mutant (YI464), and cup9Δ/Δ mutant (YI192), were serially diluted onto RPMI solid medium with or without 125 ng/ml caspofungin. Cells were incubated for 3 days at 30°C.
