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. 2021 May 25;218(4):iyab080. doi: 10.1093/genetics/iyab080

Figure 2.

Figure 2

Gαo signaling inhibits HSN neuron Ca2+ activity and burst firing. (A) Representative GCaMP5: mCherry (ΔR/R) ratio traces showing HSN Ca2+ activity in freely behaving wild-type (black), goa-1(n1134) loss-of-function mutant (green), goa-1(sa734) null mutant (blue), and egl-10(md176) null (purple) mutant animals, along with transgenic animals expressing the activated GOA-1(Q205L) in the HSN neurons (pink) during an egg-laying active state. Arrowheads indicate egg-laying events. (B) Cumulative distribution plots of instantaneous Ca2+ transient peak frequencies (and inter-transient intervals) in wild-type (black open circles), goa-1(n1134) (green filled triangles), goa-1(sa734) (blue squares), egl-10(md176) mutants (purple open squares) along with transgenic animals expressing the activated GOA-1(Q205L) in the HSN neurons (pink open circles). Asterisks indicate P < 0.0001 (Kruskal-Wallis test with Dunn’s correction for multiple comparisons). (C) Scatter plots show average Ca2+ transient frequency (per min). Error bars indicate 95% confidence intervals for the mean. Asterisk indicates P < 0.0001; pound (#) indicates P ≤ 0.0079; n.s. indicates P > 0.05 (one-way ANOVA with Bonferroni correction for multiple comparisons).