Figure 3.

Inhibitory Gα_o signaling in HSN is required for two-state Ca²⁺ activity and facilitates modulation by feedback of egg accumulation. (A) Representative GCaMP5: mCherry (ΔR/R) ratio traces showing HSN Ca²⁺ activity in untreated (fertile) wild-type animals (black open circles), FUDR-sterilized wild-type animals (black filled circles), untreated (fertile) animals expressing Pertussis Toxin (PTX) in the HSN neurons (blue open squares), and in FUDR-sterilized transgenic animals expressing PTX in the HSNs (blue filled squares). Arrowheads indicate egg-laying events. (B) Cumulative distribution plots of instantaneous Ca²⁺ transient peak frequencies (and inter-transient intervals) in untreated and FUDR-treated animals. Asterisks indicate P < 0.0001 (Kruskal-Wallis test with Dunn’s test for multiple comparisons). (C) Scatter plots show average Ca²⁺ transient frequency (per min). Error bars indicate 95% confidence intervals for the mean. Asterisks indicate P < 0.0001 (one-way ANOVA with Bonferroni’s test for multiple comparisons). Data from 10 animals were used for each strain for analysis.