Figure 6.

Altered Gα_o signaling in the vulval muscles and uv1 neuroendocrine cells causes only modest effects on egg laying. (A) Scatter plots show average number of eggs retained by wild-type (grey), goa-1(n1134) mutants (green), egl-10(md176) null mutant animals (orange) along with transgenic animals expressing GOA-1^Q205L in the HSNs (red open circles) compared to transgenic animals expressing mCherry only (pink open circles), Pertussis Toxin (blue open circles), or GOA-1^Q205L (black open circles) in the vulval muscles from the ceh-24 gene promoter. (B) Scatter plots show average number of eggs retained in transgenic animals expressing mCherry only (gray) or Tetanus Toxin along with mCherry (orange) in the vulval muscles (vm) using the ceh-24 gene promoter. Error bars indicate means with 95% confidence intervals. (C) Scatter plots show average number of eggs retained by wild-type (grey), goa-1(n1134) mutant (green), egl-10(md176) null mutant (orange) animals, and transgenic animals expressing GOA-1^Q205L in the HSNs (red) compared to transgenic animals expressing mCherry only (pink), Pertussis Toxin (blue), or GOA-1^Q205L (black open circles) in the uv1 neuroendocrine cells from the tdc-1 gene promoter. Four or five independent extrachromosomal arrays were generated for each transgene in (A–C) and ∼10 animals bearing each extrachromosomal array were analyzed. Error bars indicate 95% confidence intervals for the mean. Asterisks indicate P < 0.0001; n.s. indicates P > 0.05 (one-way ANOVA with Bonferroni’s correction for multiple comparisons or Student’s t test).