Table 1.
Strain names and genotypes for all animals used in this study (behavior assays and calcium imaging)
| Strain | Feature | Genotype | Reference |
|---|---|---|---|
| N2 | Bristol wild-type strain | Wild type | Brenner (1974) |
| DA521 | EGL-4 (protein kinase G) gain-of-function mutant. Hyperactive egg laying, sluggish locomotion | egl-4(ad805) IV | Raizen et al. (2006) |
| DG1856 | GOA-1 (Gαo) null mutant, hyperactive egg laying | goa-1(sa734) I | Robatzek and Thomas (2000) |
| IZ1236 | NLP-7 overexpression (nlp-7 promoter) | ufIs118 | Banerjee et al. (2017) |
| KG421 | GSA-1 (Gαs) gain-of-function mutant. Hyperactive locomotion | gsa-1(ce81) I | Schade et al. (2005) |
| KG518 | ACY-1 (Adenylate Cyclase) gain-of-function mutant. Hyperactive locomotion | acy-1(ce2) III | Schade et al. (2005) |
| KG532 | KIN-2 (protein kinase A inhibitory regulatory subunit) loss-of-function mutant. Hypersensitive to stimuli | kin-2(ce179) X | Schade et al. (2005) |
| KG744 | PDE-4 (phosphodiesterase) loss-of-function mutant, Hyperactive locomotion | pde-4(ce268) II | Charlie et al. (2006a) |
| LX75 | egl-10(md176) mutant strain for transgene production, multi-vulva at 20 °C in the absence of lin-15(+) rescue transgene | egl-10(md176) V; lin-15(n765ts) X | Patikoglou and Koelle (2002) |
| LX755 | HSN and NSM expressing GFP + EGL-10 (tph-1 promoter) in egl-10 mutant background. Less egg-laying defective than LX764 | egl-10(md176) V; lin-15(n765ts) X vsEx437 | This study |
| LX764 | HSN and NSM expressing GFP only (tph-1 promoter) in egl-10 mutant background. Egg-laying defective | egl-10(md176) V; lin-15(n765ts) X vsEx439 | This study |
| LX849 | HSN and NSM activated GOA-1(Q205L), tph-1 promoter, egg laying defective | vsIs49; lin-15(n765ts) X | Tanis et al. (2008) |
| LX850 | HSN and NSM Pertussis Toxin, tph-1 promoter, hyperactive egg laying | vsIs50; lin-15(n765ts) X | Tanis et al. (2008) |
| LX1832 | Strain for transgene production, blue-light insensitive, multi-vulva at 20°C in the absence of lin-15(+) rescue transgene. | lite-1(ce314) lin-15(n765ts) X | Gurel et al. (2012) |
| LX1918 | Vulval muscle GCaMP5, mCherry (unc-103e promoter, unc-54 3’ UTR) | vsIs164 lite-1(ce314) lin-15(n765ts) X | Collins et al. (2016) |
| LX1919 | Vulval muscle GCaMP5, mCherry (unc-103e promoter, unc-54 3’ UTR) | vsIs165; lite-1(ce314) lin-15(n765ts) X | Collins et al. (2016) |
| LX2004 | HSN GCaMP5, mCherry (nlp-3 promoter, nlp-3 3’UTR) | vsIs183 lite-1(ce314) lin-15(n765ts) X | Collins et al. (2016) |
| LX2007 | HSN GCaMP5, mCherry (nlp-3 promoter, nlp-3 3’UTR) | vsIs186 lite-1(ce314) lin-15(n765ts) X | Collins et al. (2016) |
| MIA26 | No HSNs | egl-1(n986dm) V | Garcia and Collins (2019) |
| MIA36 | EGL-4 (protein kinase G) gain-of-function mutant. Hyperactive egg laying, sluggish locomotion | egl-4(mg410) IV | Hao et al. (2011) |
| MIA123 | No HSNs in lite-1, lin-15 background for crossing with transgenes | egl-1(n986dm) V; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA144 | VC Tetanus Toxin (minimal lin-11 promoter enhancer, unc-54 3’UTR) | keyIs33; lite-1(ce314) lin-15(n765ts) X | Kopchock et al. (2021) |
| MIA210 | GOA-1 (Gαo) reduced-function mutant; HSN GCaMP, mCherry | goa-1(n1134) I; vsIs183 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA214 | GOA-1 (Gαo) reduced-function mutant; vulval muscle GCaMP5, mCherry | goa-1(n1134) I; vsIs164 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA216 | Increased Gαo signaling; HSN GCaMP5, mCherry | egl-10(md176) V; vsIs183 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA218 | HSN and NSM Pertussis Toxin in blue-light insensitive, lin-15 multi-vulva background | vsIs50 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA227 | HSN and NSM Pertussis Toxin (tph-1 promoter); HSN GCaMP5, mCherry (nlp-3 promoter) | vsIs186; vsIs50 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA245 | HSN and NSM Pertussis Toxin (tph-1 promoter); vulval muscle GCaMP5, mCherry (unc-103e promoter) | vsIs50 X vsIs165; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA256 | Vulval muscle mCherry (ceh-24 promoter, unc-54 3’UTR) | keyEx45; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA257 | Vulval muscle mCherry + Pertussis Toxin (ceh-24 promoter, unc-54 3’UTR) | keyEx46; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA258 | Vulval muscle mCherry + Activated GOA-1(Q205L) (ceh-24 promoter, unc-54 3’UTR) | keyEx47; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA259 | uv1 cells mCherry (tdc-1 promoter, ocr-2 3’ UTR) | keyEx48; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA260 | uv1 cells mCherry + Pertussis Toxin (tdc-1 promoter, ocr-2 3’ UTR) | keyEx49; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA261 | uv1 cells mCherry + Activated GOA-1(Q205L) (tdc-1 promoter, ocr-2 3’ UTR) | keyEx50; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA262 | Vulval muscle mCherry + Tetanus toxin (ceh-24 promoter, unc-54 3’UTR) | keyEx51; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA263 | GOA-1 (Gαo) null mutant; HSN GCaMP5, mCherry | goa-1(sa734) I; vsIs183 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA277 | Increased Gαo signaling in HSN; HSN GCaMP5, mCherry | vsIs49/+, +/vsIs183 lite-1(ce314) lin-15(n765ts) X (trans-heterozygote) | This study |
| MIA278 | HSN/NSM gpb-1 and gpc-2 overexpression + GFP (tph-1 promoter) | keyEx52; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA279 | HSN/NSM empty + GFP (tph-1 promoter) | keyEx53; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA282 | PDE-2 (phosphodiesterase) loss-of-function mutant | pde-2(qj6) | Fujiwara et al. (2015) |
| MIA291 | Increased Gαo signaling in HSN (tph-1 promoter); vulval muscle GCaMP5, mCherry (unc-103e promoter) | vsIs49; vsIs165 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA293 | NLP-7 overexpression (nlp-7 promoter); HSN GCaMP5, mCherry (nlp-3 promoter) | ufIs118; vsIs183 lite-1(ce314) lin-15(n765ts) X | Banerjee et al. (2017) and This study |
| MIA294 | NLP-7 overexpression (nlp-7 promoter); goa-1(Gαo) null mutant; HSN GCaMP5, mCherry (nlp-3 promoter) | ufIs118; goa-1(sa734) I; vsIs183 lite-1(ce314) lin-15(n765ts) X | Banerjee et al. (2017) and This stud |
| MIA295 | GOA-1 (Gαo) mutant, vulval muscle GCaMP5, mCherry (unc-103e promoter) | goa-1(sa734) I; vsIs164 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA331 | GOA-1 (Gαo) null mutant; EGL-4 (protein kinase G) null mutant | goa-1(sa734) I; egl-4(n479) IV | This study |
| MIA345 | GOA-1 (Gαo) null mutant; no HSNs | goa-1(sa734) I; egl-1(n986dm) V | This study |
| MIA359 | EGL-47 null mutant; HSN GCaMP5, mCherry (nlp-3 promoter) | egl-47(ok677) V; vsIs183 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA360 | NLP-7 overexpression (nlp-7 promoter); HSN and NSM Pertussis Toxin (tph-1 promoter); HSN GCaMP5, mCherry (nlp-3 promoter) | ufIs118; vsIs50; vsIs186 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA366 | NLP-7 overexpression [nlp-7 promoter; EGL-47 null mutant; HSN GCaMP5, mCherry (nlp-3 promoter)] | ufIs118; egl-47(ok677) V; vsIs183 lite-1(ce314) lin-15(n765ts) X | This study |
| MIA369 | No HSNs; HSN and NSM Pertussis Toxin (tph-1 promoter); HSN GCaMP5, mCherry (nlp-3 promoter) | egl-1(n986dm) V; vsIs50; vsIs186 lite-1(ce314) lin-15 (n765ts) X | This study |
| MIA370 | VC Tetanus Toxin (lin-11 enhancer/promoter); HSN and NSM Pertussis Toxin (tph-1 promoter); HSN GCaMP5, mCherry (nlp-3 promoter) | keyIs33; vsIs186; vsIs50 keyIs33; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA371 | GOA-1 (Gαo) null mutant; VC Tetanus Toxin (lin-11 enhancer/promoter) | goa-1(sa734) I; keyIs33; lite-1(ce314) lin-15(n765ts) X | This study |
| MIA425 | HSN Pertussis Toxin rescued with HSN GOA-1Q205L (tph-1 promoter) | keyEx82; vsIs50; lin-15(n765ts) | This study |
| MT1073 | EGL-4 (protein kinase G) loss-of-function mutant. Egg-laying defective, roaming locomotion | egl-4(n478) IV | L'Etoile et al. (2002) |
| MT1074 | EGL-4 (protein kinase G) null mutant. Egg-laying defective, roaming locomotion | egl-4(n479) IV | L'Etoile et al. (2002) |
| MT2426 | GOA-1 (Gαo) reduced-function mutant, hyperactive egg laying | goa-1(n1134) I | Segalat et al. (1995) |
| MT8504 | Increased Gαo signaling due to mutation in RGS protein, EGL-10 | egl-10(md176) V | Koelle and Horvitz (1996) |
| RB850 | EGL-47 null mutant | egl-47(ok677) V | Moresco and Koelle (2004) and C. elegans Deletion Mutant Consortium (2012) |