Fig. 10.

Combination treatment affected expression of proteins and genes involved in metabolism regulation. A Effects of combination treatment on protein expression of G6PD, AKT, AKT2, and c-Myc in PC/DX25 cells treated with 25 nM docetaxel plus increasing concentration of CAPE (0, 10, 20, 40 μM) for 96 h were examined by Western blotting. The β-actin was used as loading control. Effects of combination treatment on expression of metabolic genes in PC/DX25 cells (B) and DU/DX50 cells (C) being treated with 25 nM or 50 nM docetaxel, respectively, along with increasing concentration of CAPE (0, 10, 20, 40 μM) for 48 h were examined by qRT-PCR. Genes with change larger than 2 folds were shown by heatmap using log₂ values