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. 2022 Feb 23;29:16. doi: 10.1186/s12929-022-00797-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Combination treatment affected the expression of apoptosis regulatory proteins in PC/DX25 and DU/DX50 cells. PC/DX25 (A) and DU/DX50 (B) cells were treated with 25 nM or 50 nM docetaxel, respectively, in combination with 0, 20, or 40 μM CAPE for 96 h. Protein expression of PARP, Bcl-2, Bax, caspase 3, cleaved caspase 3, caspase 9, cleaved caspase 9, caspase 8, cyclin D1, AVEN, and cytochrome c was examined by Western blotting. The β-actin was used as loading control. Quantification for protein expression level of important regulatory proteins from at least three different experiments was shown for PC/DX24 (C) and DU/DX50 (D) cells