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. 2021 Sep 7;21(21):23737–23750. doi: 10.1109/JSEN.2021.3110857

TABLE I. Nucleic Acid-Based Test.

Ref. Substrate / Approach Detection tools Detection time LOD
[42] RT-LAMP Colorimetry 1 h -
[102] One-step RT-LAMP Colorimetry 20 min 80 copies/ml
[103] RT-LAMP Colorimetry 20 min 100 copies/ Inline graphic
[104] One-step RT-LAMP Colorimetry 30 min 1000 copies/ml (5 copies)
[105] RT-LAMP Colorimetry 40 min -
[106] RT-LAMP Fluorescent >15 min 1 Inline graphic 101 – 1 Inline graphic 102 copies/reaction
[107] RT-LAMP Colorimetry 30 min 102copies
[108] RT-LAMP Colorimetry, spectrophotometry, and real time fluorescence >1 h 59 copies/reaction
[109] RT-LAMP Colorimetry 45 min 200 virions/ Inline graphic
[110] Isothermal rolling circle amplification (RCA) Portable potentiostat >2 h 1 copy/ Inline graphic
[17] Thiol-modified ssDNA-capped AuNPs, paper-based Simple signal conditioning circuit >5 min 6.9 copies/ Inline graphic
[111] Supersandwich-type recognition strategy EIS - 200 copies/ mL (clinics)
[47] CRISPR-based SHERLOCK Fluorescent and colorimetric readout >1 hour 2 aM
[46] Recombinase polymerase amplification (RPA) LightMix®Modular - -
[49] Combined isothermal amplification with CRISPR–Cas12 DETECTR technology fluorescence-based readout <40 min 10 copies per Inline graphic
[102] One-step RT-LAMP Colorimetry 20 min 80 copies/m
[112] Two-step RT-LAMP fluorescence-based readout 25 min 2 copies per Inline graphic
[44] RT-LAMP assay on semiconductor technology Electrochemical >20min 10 copies/reaction

RT-LAMP = reverse transcription loop-mediated isothermal amplification, ssDNA = single-stranded DNA, AuNPs = gold nanoparticles, CRISPR = clustered regularly interspaced short palindromic repeats, SHERLOCK = Specific High Sensitivity Enzymatic Reporter Unlocking, DETECTR = DNA Endonuclease-Targeted CRISPR Trans Reporter.