Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Human insulin | Eli Lilly | NDC 0002–8215-17 (Humulin R U-100) |
| TRI reagent | Sigma | T9494 |
| SYBR Green | Thermo Fisher Scientific | 4309155 |
| DreamTaqDNA Polymerase | Thermo Fisher Scientific | EP0705 |
| Superscript III Reverse Transcriptase | Thermo Fisher Scientific | 18080085 |
| Critical commercial assays | ||
| Ultra-sensitive mouse insulin ELISA | Crystal Chem | Cat# 90080 |
| Mouse/Rat FGF-21 Quantikine ELISA Kit | R&D Systems | Cat# MF2100 |
| Deposited data | ||
| Raw and analyzed RNAseq data | This paper | GEO: GSE181301; Table S5 |
| Lipidomics | This paper | Table S9 |
| Metabolomics | This paper | Table S11 |
| Experimental models: Organisms/strains | ||
| Mouse strain: C57BL6/J (Male and Female) | The Jackson Laboratory | Cat# JAX:000664; RRID: IMSR_JAX:000664 |
| Mouse strain: DBA2/J (Male and Female) | The Jackson Laboratory | Cat# JAX: 000671; RRID: IMSR_JAX:000671 |
| Mouse strain: UM-HET3 (Male and Female) | This paper | F2 progeny of (BALB/cJ x C57BL/6J) mothers and (C3H/HeJ x DBA/2J) fathers |
| Mouse strain: BALB/cJ (Female) | The Jackson Laboratory | Cat# JAX: 000651; RRID: IMSR_JAX:000651 |
| Mouse strain: C3H/HeJ (Female) | The Jackson Laboratory | Cat# JAX: 000659; RRID: IMSR_JAX:000659 |
| Mouse strain: C57BL6/J.Nia (Male and Female) | NIA Aged Rodent Colony | C57BL/6J.Nia |
| Mouse strain: Fgf21loxP/loxP | The Jackson Laboratory (Schwenk et al., 1995), (Potthoff et al., 2009) | Cat# JAX:022361; RRID: IMSR_JAX:022361 |
| Mouse strain:C57BL/6J; Fgf21Δ/Δ | Lamming Laboratory (Yu et al., 2021) | N/A |
| Oligonucleotides | ||
| Mouse FGF21 genotyping WT F: ACCCCCTGAGCATGGTAGA |
The Jackson Laboratory | 51425, https://www.jax.org/Protocol?stockNumber=033846&protocolID=37478 |
| Mouse FGF21 genotyping mutant F: CAGACCAAGGAGCACAGACC |
The Jackson Laboratory | 51434, https://www.jax.org/Protocol?stockNumber=033846&protocolID=37478 |
| Mouse FGF21 genotyping common R: GCAGAGGCAAGTGATTTTGA |
The Jackson Laboratory | 51435, https://www.jax.org/Protocol?stockNumber=033846&protocolID=37478 |
| Mouse Cre genotyping common F: GAACCTGATGGACATGTTCAGG |
(Yu et al., 2019) | N/A |
| Mouse Cre genotyping common R: AGTGCGTTCGAACGCTAGAGCCTGT |
(Yu et al., 2019) | N/A |
| Actb: F: ACCTTCTACAATGAGCTGCG | (Fontana et al., 2016) | N/A |
| Actb: R: CTGGATGGCTACGTACATGG | (Fontana et al., 2016) | N/A |
| Fgf21: F: CAAATCCTGGGTGTCAAAGC | (Fontana et al., 2016) | N/A |
| Fgf21: R: CATGGGCTTCAGACTGGTAC | (Fontana et al., 2016) | N/A |
| Software and algorithms | ||
| edgeR package | (Robinson et al., 2010) | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| GraphPad Prism | GraphPad | http://www.graphpad.com/scientific-software/prism |
| MetaboAnalyst | (Chong et al., 2019) | https://www.metaboanalyst.ca/ |
| R (Version 3.4.3) | N/A | https://www.r-project.org/ |
| MyGene package | N/A | https://www.bioconductor.org/packages/release/bioc/html/mygene.html |
| Factoextra package | (Kassambara and Mundt, 2020) | https://cran.r-project.org/web/packages/factoextra/index.html |
| missMDA package | N/A | https://cran.r-project.org/web/packages/missMDA/index.html |
| LION/web | (Molenaar et al., 2019) | http://www.lipidontology.com/ |
| Limma package | (Ritchie et al., 2015) | https://bioconductor.org/packages/release/bioc/html/limma.html |
| Other | ||
| Normal Chow | Purina | Cat# 5001 |
| Mouse diets (See Table S1) | This paper | N/A |