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. 2022 Feb 21;11(1):616–628. doi: 10.1080/22221751.2022.2037393

Figure 3.

Figure 3.

ZNF143 is a direct target of miR-192-3p. (A) HepG2.2.15 cells were transfected with different specific siRNAs or negative control (siNC) at 40 nM; Huh7 cells were co-transfected with pSM2 plasmid and different siRNAs or siNC at 40 nM, and harvested at 72 h post-transfection. Secreted HBsAg level in culture supernatants was determined by chemiluminescence immunoassay. (B) Schematic diagram of miR-192-3p-binding sites within the consequences of ZNF143 mRNA 3′UTR. (C) HepG2.2.15 cells were co-transfected with miR-192-3p mimics or miNC at 40 nM and ZNF143 luciferase reporter plasmid containing its 3′UTR nucleotide consequences with Renilla as an internal control. The data from dual-Glo luciferase report assay were calculated by fold change and normalized to the miNC samples. (D–F) HepG2.2.15 cells were transfected with miR-192-3p mimics or inhibitors at 40 nM and harvested at 72 h post-transfection. ZNF143 mRNA (D) and protein (E) levels were measured by quantitative real-time RT-PCR and Western blotting analysis, separately. (F) Secreted HBsAg level in culture supernatants was determined as described above. *P < .05; **P < .01; ns, no significance.