Table 1.
Primers used in the study for real-time qPCR and size of PCR products.
| Target organism | Primer Sequence (5⇒ to 3⇒) | PCR product bp | T°C | Reference |
|---|---|---|---|---|
| Lactobacillus group |
F: TGGAAACAGRTGCTAATACCC R: GTCCATTGTGGAAGATTCCC |
200 | 55 | Frank et al. 2008 |
| Bifidobacterium |
F: GCGTGCTTAACACATGCAAGTC R: CACCCGTTTCCAGGAGCTATT |
126 | 60 | Yadav et al. 2013 |
| Bacteroides | F: GTCAGTTGTGAAAGTTTGC R: CAATCGGAGTTCTTCGTG |
127 | 60 | Ahmed et al. 2007 |
| Firmicutes |
F: AGYATGTGGTTTAATTCGAAGCA R: AGCTGACGACAACCATGCAC |
126 | 60 | Yadav et al. 2013 |
| All bacteria | F: ACTCCTACGGGAGGCAGCAGT R: GTATTACCGCGGCTGCTGGCAC |
200 | 60 | Ahmed et al. 2007 |