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. 2022 Feb 20;14(1):2035661. doi: 10.1080/19490976.2022.2035661

Figure 3.

Figure 3.

Stress-induced modifications to IECs are dependent on an intact gut microbiota. RT-PCR was used to analyze the expression of a select set of genes representing ROS-generating (Duox2, Nos2), mucus regulating (Fut2), innate inflammatory (Saa1, Il18) and antimicrobial (Reg3b,), signaling pathways within IECs (EpCAM+ CD45-) isolated from (a) conventionally-raised (b) germ-free and (c) broad spectrum antibiotic treated C57Bl/6N mice exposed to Con or Str conditions. n = 6–9/group; ns = not significant at p > .05, * = p < .05, ** = p < .01, *** = p < .001. nd = not detected.