Figure 4.

Phylogenetic analysis of TEX264 proteins. TEX264 orthologs in vertebrates are highlighted in orange (invertebrates are colorless) and bacterial GyrI-domain containing proteins are highlighted in blue. Ftsa proteins were used as an outgroup (Fig. S2). Full-length protein sequences were aligned with the MAFFT alignment algorithm. The phylogenetic tree was constructed using the maximum likelihood method. The expanded phylogenetic tree with detailed methodology is shown in Fig. S2.