Figure 2.

CAR T cells exhibit longitudinal plasticity in vitro.

(A) Experimental workflow (n = 3 healthy donors). (B) Normalized (99.9th percentile) violin plots of all assessed proteins across CAR T cells longitudinally. Black dots indicate population means. Bimodality indicated by arrows. *p < .05, **p < .01, ***p < .001 and ****p < .0005 indicate significant difference. (C) Normalized (99.9th percentile) median expression of all assessed proteins across CAR T cells longitudinally. (D) Overlay of unsupervised clustering by FlowSOM of CAR T cells on optSNE embedding, separated longitudinally. Each color designates a cluster ID. (E) Pie charts of mean cluster abundance longitudinally. Colors correspond to the clusters shown in the optSNE embedding in panel D. (F) Abundance of each of the 16 clusters determined by FlowSOM clustering. (G) Normalized (99.9th percentile) median expression of all assessed proteins across CAR T cells clusters. ^non-CAR T cells. PMBCs = peripheral blood mononuclear cells; PS = post stimulation