FIG. 3.

PFGE banding patterns of the small colonial variant of an isolate of VREM following 20 serial single colony subcultures. (Gel A) Linear ramped pulse time of 10 to 40 s with a run time of 40 h. (Gel B) Linear ramped pulse time of 1 to 10 s with a run time of 40 h. Lanes 1 and 7, original culture; lanes 2 and 8, 1st subculture; lanes 3 and 9, 5th subculture; lanes 4 and 10, 10th subculture; lanes 5 and 11, 15th subculture; lanes 6 and 12, 20th subculture. Arrows, bands which appeared and/or disappeared during subculture; dashed line, the positions of the same band on both gels.