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PFGE of total DNA and plasmid preparation by using ramped pulse times of 1 to 10 s for 30 h followed by 10 to 30 s for 15 h. Lanes 1 to 3, isolate with PFGE pattern 4a; lanes 4 to 6, isolate with PFGE pattern 4c; lanes 1 and 4, undigested plasmid preparation; lanes 2 and 5, plasmid preparation digested with SmaI; lanes 3 and 6, total DNA digested with SmaI. Arrow, plasmid band.
