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Rosette-like cell aggregate formation in siRNA-treated culture. (a) Culture scheme for establishing neural rosettes. (b) RT-qPCR assay to examine the effect of siRNA knock-down. *: P < 0.05. (c) Immuno-staining of PAX6 and N-cadherin on cells. (d) RT-qPCR assay to examine the expression of PAX6 in each group. *: P< 0.05.
