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. 2021 May 9;18(1):171–190. doi: 10.1080/15548627.2021.1922982

Figure 6.

Figure 6.

Loss of UBXN1 impacts mitochondrial recruitment of VCP. (A) HeLa (WT), HeLa UBXN1-/- clone 22 (#22) and clone 71 (#71) cells transfected with an expression plasmid for mitoYFP-T2A-MYC-PRKN, treated with 25 µM CCCP for 90, 180, 360 min or left untreated, were fixed, stained using mouse anti-VCP antibodies and imaged by confocal microscopy. Shown are representative images from two independent experiments after 90, 180, and 360 min of CCCP treatment. Scale bar: 20 µm. (B) Mitochondrial translocation of VCP in HeLa was quantified by line drawings across mitochondria of wild-type and UBXN1-/- clone #22 and #71 cells. Shown is the mean Pearson’s correlation between VCP and mitoYFP of 28 to 125 cells per condition from two independent experiments. Statistical significance was tested using unbalanced two-way ANOVA followed by Student’s t-test with “fdr” adjustment for multiple testing.