Figure 11.

Ectopic expression of the mitochondrial VCP cofactor UBXN6 promotes PRKN translocation in cells lacking UBXN1. (A) HeLa cells (WT) and cells of HeLa UBXN1^-/- clone 22 (#22) and clone 71 (#71) were transfected with expression plasmids for FLAG-UBXN6 or control vector, mitoYFP and mCherry-PRKN, treated with 25 µM CCCP for 8 h, fixed, stained using rabbit anti-FLAG antibodies and imaged by confocal microscopy. Shown are representative images from two independent experiments. The overlay panel displays mitoYFP and mCherry-PRKN. Scale bar: 20 µm. (B) Distribution of mitoYPF and mCherry-PRKN staining was measured by line drawing. Shown are boxplots of Pearson’s correlations of two independent experiments with 18 to 33 cells per condition. Statistical significance was assessed by unbalanced two-way ANOVA with Student’s t-test applying “fdr” correction to account for multiple testing.