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. Author manuscript; available in PMC: 2022 Dec 17.
Published in final edited form as: ACS Catal. 2021 Nov 30;11(24):14956–14966. doi: 10.1021/acscatal.1c04520

Fig 2. Determination of the substrate specificity of 3-OST-5 using 8-mer substrates.

Fig 2.

A) Sulfation reaction carried out by 3-OST-5 using 8-mer-1, 8-mer-2 and 8-mer-3 substrates. The structures of the 8-mers are presented in shorthand symbols. The key for the shorthand symbols is presented (right). Red dots in the structures represent sites with 13C-carbon. B) Structural analysis of 13C-labeled 8-mer-4 using the LC-MS/MS method. The schematic digestion of 8-mer by heparin lyases is presented (top). The digested fragments (a-c, diagrammed on right) were then assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Spectrum analysis allowed for matching of the product to a particular structure based on observed mass peaks.