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. 2022 Feb 23;10(1):e01858-21. doi: 10.1128/spectrum.01858-21

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Copyright © 2022 Zhang et al.

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FIG 3 — Overexpression of rsmA/rsmN in the ΔnrtR mutant restored the expression of H1-T6SS. (A, B) The relative hcp1 mRNA levels in PAK and ΔnrtR containing pUCP20 (empty vector, EV), pUCP20-rsmN, or pUCP20-rsmA. Total RNA was isolated from bacteria at an OD₆₀₀ of 1.0, and hcp1 mRNA levels were examined by real-time qPCR using rpsL as an internal control. **, P < 0.01; ***, P < 0.001, by Student's t test. (C) PAK and ΔnrtR integrated with an hcp1-Flag driven by its native promoter containing pUCP20 (empty vector, EV), pUCP20-rsmN (rsmN), or pUCP20-rsmA (rsmA) were grown to an OD₆₀₀ of 1.0 in LB medium. Proteins from an equivalent number of P. aeruginosa cells of the indicated strains were separated on a 12% SDS-PAGE and probed with an antibody against Flag or RpoA.