TABLE 1.
Organic acid concentrations (mM ± standard deviation) at 48 h for the wild-type (WT) and various mutant strains
| Strain | Acetate | α-keto glutarate | Formate | Lactate | Malate | Propionate | Pyruvate | Succinate |
|---|---|---|---|---|---|---|---|---|
| WT | 15.78 ± 0.52 | 0.02 ± 0.00 | 8.81 ± 0.19 | 9.37 ± 0.42 | 2.03 ± 0.12 | 4.69 ± 0.34 | 0.10 ± 0.00 | 8.66 ± 0.15 |
| ΔBT2955 | 15.42 ± 0.37 | 0.02 ± 0.01 | 8.10 ± 0.43 | 9.74 ± 0.55 | 2.28 ± 0.13 | 4.45 ± 0.02 | 0.13 ± 0.02 | 8.46 ± 0.12 |
| ΔBT4738 | 14.87 ± 0.40 | 0.01 ± 0.00 | NDa** | 6.90 ± 0.72 | 2.27 ± 0.16 | 5.20 ± 0.32 | 0.22 ± 0.03 | 11.07 ± 0.16** |
| ΔBT2955 /4738b |
15.61 ± 1.17 | 0.01 ± 0.00 | ND** | 6.63 ± 0.33* | 2.37 ± 0.06 | 5.47 ± 0.83 | 0.19 ± 0.00** | 11.25 ± 0.73 |
| ΔBT1575 | 16.19 ± 0.64 | 0.02 ± 0.02 | 9.14 ± 0.69 | 7.69 ± 0.73 | 1.88 ± 0.33 | 5.21 ± 0.65 | 0.14 ± 0.07 | 8.73 ± 0.70 |
| ΔBT4455-7 | 15.99 ± 0.34 | 0.02 ± 0.00 | 8.62 ± 0.81 | 9.76 ± 0.19 | 2.26 ± 0.11 | 4.64 ± 0.15 | 0.14 ± 0.03 | 8.82 ± 0.53 |
| ΔBT1575 /4455-7 |
16.59 ± 0.74 | 0.02 ± 0.02 | 9.60 ± 0.91 | 8.28 ± 0.45 | 1.86 ± 0.44 | 4.97 ± 0.60 | 0.17 ± 0.05 | 8.90 ± 0.32 |
| ΔBT1450 | 16.48 ± 0.70 | 0.01 ± 0.00 | 9.17 ± 0.39 | 8.27 ± 0.72 | 1.78 ± 0.17 | 5.23 ± 0.60 | 0.12 ± 0.02 | 8.03 ± 0.33 |
| ΔBT1686-9 | 16.25 ± 0.39 | 0.02 ± 0.01 | 7.92 ± 0.14* | 9.31 ± 0.62 | 2.08 ± 0.31 | ND* | 0.12 ± 0.02 | 15.00 ± 0.27** |
| ΔBT1917 | 16.07 ± 0.02 | 0.02 ± 0.01 | 9.09 ± 0.34 | 9.24 ± 0.65 | 2.07 ± 0.22 | 5.01 ± 0.22 | 0.14 ± 0.00** | 8.10 ± 0.31 |
| ΔBT2090-1 | 15.77 ± 0.29 | 0.06 ± 0.01 | 8.75 ± 0.49 | 8.98 ± 1.08 | 1.88 ± 0.76 | ND* | 0.17 ± 0.02 | 14.77 ± 0.60* |
| ΔBT3193 | 1.72 ± 0.25** | ND | ND** | 2.73 ± 0.40** | ND* | ND* | 1.17 ± 0.35 | 0.74 ± 0.35** |
| ΔBT1969 | 14.61 ± 0.94 | 0.03 ± 0.01 | 7.23 ± 0.61 | 8.75 ± 1.10 | 2.37 ± 0.31 | 5.32 ± 1.01 | 0.14 ± 0.02 | 10.40 ± 0.49 |
| ΔBT1820 | 17.04 ± 0.61 | 0.01 ± 0.01 | 8.30 ± 1.29 | 8.44 ± 0.46 | 2.01 ± 0.27 | 5.27 ± 0.39 | 0.15 ± 0.01** | 8.42 ± 0.91 |
| ΔBT1822 | 16.04 ± 1.14 | 0.01 ± 0.00 | 9.47 ± 0.49 | 8.63 ± 0.56 | 1.75 ± 0.13 | 5.16 ± 0.54 | 0.15 ± 0.04 | 8.08 ± 0.60 |
| ΔBT1820 /1822 |
16.00 ± 0.35 | 0.02 ± 0.01 | 8.93 ± 0.67 | 9.33 ± 0.26 | 2.04 ± 0.21 | 4.72 ± 0.19 | 0.11 ± 0.00 | 9.09 ± 0.59 |
a
ND: Not detected; butyrate and fumarate were not detected in any samples.*, P < 0.05; **, P < 0.01 according to Welch two-sample t test adjusted for multiple comparisons with the Benjamini/Hochberg FDR method.
b
n = 2; all others n = 3.