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Analysis of Env-receptor affinity through ELISA. (a) Western blot analysis of the soluble chNHE1-ECL1-rIgG. (b) Detection of affinity of various mutant Env expression cell lysates with NHE1-ECL1-rIgG using ELISA. The binding capability was measured by OD450 values of negative DF-1 cells, wide-type, N17, N62, and N193.
