TABLE 1.
Summary of identified NGSs and glycan chains on ALV-J gp85 protein through LC-MS
| N-linked glycosylation sites | Identified peptides | Peptide no. | Type of N-glycan chains |
Avg mass of N-glycan chain | ||
|---|---|---|---|---|---|---|
| High-mannose form | Hybrid form | Complex form | ||||
| N17 | KSSCSVGM1GVHLLQQPGNVWVTWANKT19 | 67 | 0.00% (2) | 16.67% (12) | 83.33% (53) | 2906.12 |
| N98 | 97KN*GTKRTCVTFGSVCYKE114 | 299 | 3.68% (11) | 6.02% (18) | 90.30% (270) | 2276.83 |
| N116 | 102RTCVTFGSVCYKENN*RS118 | 222 | 2.25% (5) | 9.91% (22) | 87.84% (195) | 2333.51 |
| N131 | 121RVCHIFDGNFN*GTGGAEAELRD142 | 332 | 4.22% (14) | 14.46% (48) | 81.33% (270) | 1915.74 |
| N157 | 146KWKGNDHLIRPYVN*QSWTMVSPINTESFSISSRY179 | 451 | 9.31% (42) | 13.75% (62) | 76.94% (347) | 2898.52 |
| N185 | 178RYCGFTSN*ETRY189 | 95 | 7.37% (7) | 17.89% (17) | 74.74% (71) | 1664.23 |
| N193 | 188RYYSGN*YSDWCSSKG202 | 159 | 3.77% (6) | 15.09% (24) | 81.13% (129) | 1958.03 |
| N209 | 201KGGEWSGGN*CTAEWNYYAYGFTFRT225 | 134 | 4.48% (6) | 14.93% (20) | 80.60% (108) | 1867.90 |
| N226 | 224RTN*ESEVLWNNGTAKA239 | 12 | 33.33% (4) | 25.00% (3) | 41.67% (5) | 2478.41 |
| N234 | 224RTNESEVLWNN*GTAKA239 | 2 | 0.00% (0) | 0.00% (0) | 100.00% (2) | 1939.18 |
| N274 | 258RNALGGPCYLGQLTMLSPN*FTTWMTYGPNITGHRR292 | 47 | 25.53% (12) | 72.34% (34) | 2.12% (1) | 1438.75 |
| N284 | 258RNALGGPCYLGQLTMLSPNFTTWMTYGPN*ITGHRR292 | 68 | 5.88% (4) | 22.06% (15) | 72.06% (49) | 1814.13 |
| Total | 1888 | 5.99% (113) | 14.56% (275) | 79.45% (1500) | 2267.54 | |
The italicized portion of the sequence represents signal peptide ahead gp85.
N* represents identified N-glycosylation sites from LC-MS.
Superscript numbers represent positions of amino acids in gp85-rIgG.