FIG 7.

CHIKV K211E enhances dissemination in A. aegypti mosquitoes. (A and B) Infectious titers quantified in bodies (A) and legs and wings (B) of A. aegypti mosquitoes infected with wild-type CHIKV or E1 variants (IOL V156A, N = 45; IOL-K211T, N = 42; IOL V156A+K211T, N = 46). Seven-day-old A. aegypti mosquitoes were fed an artificial blood meal containing virus (10⁶ PFU/ml) and incubated for 14 days. Viral titers were determined for homogenized mosquito bodies or legs/wings by plaque assay. (C) Percent dissemination was determined as the percentage of mosquitoes for which titers were detected in the body as well as legs and wings. Data represent two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Kruskal-Wallis test.