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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: J Thromb Haemost. 2021 Jul 23;19(10):2417–2427. doi: 10.1111/jth.15456

FIGURE 1.

FIGURE 1

The impact of the method of blood collection on the parameters determined by rotational thromboelastometry (ROTEM). Blood samples were collected via tail bleed (TB), submandibular bleed (SB), eye bleed (EB), or inferior vena cava (IVC) blood draw from factor VIII knockout (FVIIInull) or wild-type (WT)-C57BL/6J mice; 3.8% sodium citrate was used as an anticoagulant at 1:10 (vol/vol). The hemostatic properties of blood samples were analyzed by ROTEM using the MiniCup, in which 7 μl 0.2 M CaCl2 was loaded to the bottom, followed by adding 105 μl of citrated blood and parameters were recorded for 1 h. A, Clotting time. B, Clot formation time. C, Alpha angle (α-angle). *P < .05. **P< .01. ***P < .001. ****P < .0001