Fig 2. Motif discovery analysis of 19 bacterial GAD proteins and 3 animal GAD65 proteins to identify motifs conserved across various species and their functional relevance with GAD protein.

Across all 22 GAD sequences under consideration, 10 motifs were each found in at least 10 of the sequences used for motif discovery. Each motif appears in a separate color below with the individual sequences described as well. Furthermore, the GAD sequences have been grouped based on the organism category from Table 1, and the respective categories have been represented as colored bars next to the organism name. In addition, 2 of the 10 motifs were found in all of the sequences. Each motif was then queried for using Pfam and CDD searches, and it was found that all 10 of the motifs hit the PDD domain in the GAD65 protein. All motifs match the pyridoxal-dependent decarboxylase (PDD) conserved domain.