Table 1. Primer and probes used to detect B. anthracis in two outbreaks of anthrax in Amhara region, northern Ethiopia, 2018-2019. Primers were supplied by the United States Centers for Disease Control and Prevention.

Primer/probe	Sequence (5’-3’)	Target gene	Reference
PA-forward	CGG ATC AAG TAT ATG GGA ATA TAG CAA	pagA	[50]
PA-reverse	CCG GTT TAG TCG TTT CTA ATG GAT	pagA	[50]
PA-probe	6FAM-CTC GAA CTG GAG TGA AGT GTT ACC GCA AAT-BHQ1	pagA	[50]
CAP-forward	ACG TAT GGT GTT TCA AGA TTC ATG	capB	[50]
CAP-reverse	ATT TTC GTC TCA TTC TAC CTC ACC	capB	[50]
CAP-probe	6FAM-CCA CGG AAT TCA AAA ATC TCA AAT GGC AT-BHQ1	capB	[50]
RNP-forward	AGA TTT GGA CCT GCG AGC G	RNase P	[51]
RNP-reverse	GAG CGG CTG TCT CCA CAA GT	RNase P	[51]
RNP-probe	6FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ1	RNase P	[51]

Probes were labeled at the 5’-end with the reporter molecule 6-carboxyfluorescein (6FAM) and at the 3’- end with the quencher, Black Hole Quencher-1 (BHQ1).