Fig 3. Both suppressor mutations overlap with the longest stretch of identity in PRCC.

Multiple sequence alignment of PRCC-1 and orthologs. The “proline-rich” region frequently observed in human oncogenic fusions is indicated in gray, and all prolines are highlighted in gray, the suppressor mutation I371 is highlighted in yellow, the suppressor deletion (Δ289–377) is indicated in red. Sequence conservation is annotated as described in the key. PRCC(null) is not represented because it is a deletion of all coding regions of the gene. Vertical blue arrows mark the commonest breakpoints for PRCC N-terminal oncogenic fusions [74]. Alignment generated in Clustal Omega [97].