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. 2022 Feb 10;18(2):e1010028. doi: 10.1371/journal.pgen.1010028

Table 2. Genotypes of C. elegans strains used in this study.

Strain Name Allele Names Allele Descriptions
N2 wild-type isolate
SZ181 unc-73(e936) /G/UU cryptic 5’ splice site uncoordinated strain
SZ283 unc-73(e936)dxbp-1(az105)I Suppressor of unc-73(e936), KIN17(K23N)
SZ162 unc-73(e936)dxbp-1(az33)I Suppressor of unc-73(e936), KIN17(M107I)
SZ280 unc-73(e936)I;prcc-1(az102)IV Suppressor of unc-73(e936), PRCC(I371F)
SZ281 unc-73(e936)I;prcc-1(az103)IV Suppressor of unc-73(e936), PRCC(Δ298–377)
SZ219 unc-73(az63)I CRISPR mimic of unc-73(e936)
SZ391 unc-73(az63)dxbp-1(az121)I;dpy-10(cn64)II CRISPR mimic of unc-73(e936) and dxbp-1(az105)(K23N)
SZ222 unc-73(az63)dxbp-1(az52)I CRISPR mimics of unc-73(e936) and dxbp-1(az33), KIN17(M107I)
SZ308 unc-73(e936)I;prcc-1(az122)IV Suppressor of unc-73(e936), CRISPR mimic PRCC(I371F)
SZ348 unc-73(e936)I; prcc-1(gk5556)IV gk5556 is deletion of all coding region of prcc-1, PRCC(null)
SZ325 dxbp-1(az137)I/hT2 I,III Deletion of KIN17(null)/HT2 over GFP balancer
SZ159 unc-73(e936az30)I Intragenic suppressor of unc-73(e936) (doublet only) 2-Choice
SZ300 unc-73(e936az30)dxbp-1(az121)I unc-73(e936az30) background, CRISPR mimic KIN17(K23N)
SZ224 unc-73(e936az30)dxbp-1(az52)I unc-73(e936az30) background, CRISPR mimic KIN17(M107I)
SZ301 unc-73(e936az30)I;prcc-1(az122)IV unc-73(e936az30) background, CRISPR mimic PRCC(I371F)
SZ263 unc-73(az100)I unc-73 CRISPR-engineered reporter construct (doubled doublet)
SZ324 unc-73(az100)dxbp-1(az121)I doubled doublet unc-73 with KIN17(K23N)
SZ310 unc-73(az100)dxbp-1(az52) I doubled doublet unc-73 with KIN17(M107I)
SZ320 unc-73(az100)I; prcc-1(az122)IV doubled doublet unc-73 with PRCC(I317F)
SZ340 smg-4(az152)V CRISPR null allele of smg-4
SZ345 unc-73(e936az30)dxbp-1(az121)I;smg-4(az152)V NMD mutant, CRISPR mimic KIN17(K23N)
SZ355 unc-73(az63)dxbp-1(az52)I; smg-4(az152)V NMD mutant, CRISPR mimic KIN17(M107I)
SZ346 prcc-1(az122)IV; smg-4(az152)V NMD mutant, CRISPR mimic PRCC(I371F)
SZ356 prcc-1(gk5556)IV; smg-4(az152)V NMD mutant, PRCC(null)
VC4596 dxbp-1(gk5666[loxP+Pmyo-2::GFP::unc-54 3’UTR + Prps-27::neoR::unc-54 3’ UTR + loxP])/+ I. Gene Knockout Consortium Heterozygous dxbp-1 deletion
VC4484 prcc-1(gk5556[loxP+myo-2p::GFP::unc-54 3’UTR + rps-27p::neoR::unc-54 3’ UTR + loxP]) IV. Gene Knockout Consortium homozygous prcc-1 deletion