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. 2021 Mar 30;204(12):1418–1432. doi: 10.1164/rccm.202102-0329OC

Figure 5.

Figure 5.

Transcriptomic reprogramming by mesenchymal stem/stromal cell–small extracellular vesicles (MEx) promote an immunosuppressive monocytic phenotype. (A) Poly-A capture RNA sequencing of isolated bone marrow–derived monocytes (BMDMono) was performed on control (BMDMono) and MEx-educated (BMDMono + MEx) groups, n = 3 per group. The heat map shows the differentially expressed genes (blue [0] to red [10] through white) ranked according to adjusted P value. (B) Principal component analysis (PCA) between control (BMDMono) and MEx-educated (BMDMono + MEx) groups. (C) Validation of candidate RNA seqencing markers was assessed by qRT-PCR in independent (separate) experiments. Data are shown as median (minimum–maximum). n = 3–6 per group; **P < 0.01.