Figure 6.
Mesenchymal stem/stromal cell–small extracellular vesicles (MEx) modulate the epigenetic landscape of bone marrow–derived monocytes (BMDMono). BMDMono were isolated and characterized as described in text. Transposase-accessible chromatin with high-throughput sequencing (ATAC seq) is a method for mapping genome-wide chromatin accessibility. Upstream promoter regions for candidate markers were obtained using the University of California Santa Cruz genome browser, with a search radius of 50,000 bp upstream. IGV software was used to overlay and visualize ATAC seq peak calls and representative RNA sequencing (RNA seq) expression for (A) CCR2, (B) CCL2, (C) IL10, and (D) ARG1. Purple peaks = BMDMono; turquoise peaks = MEx-educated BMDMono. Peak heights were set at 100 for ATAC seq and 80 for RNA seq. (E) Respective quantification of upstream promoter region peak calls. n = 3 per group; *P < 0.05.