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SgRNA sequences used. Common oligo taken from (Nakayama et al., 2013).
| SgRNA (sgRNA) Oligo | Sequence 5’ to 3’ |
|---|---|
| sg219-5 | taatacgactcactataGGTGAATTTTCCACAGCAATgttttagagctagaa |
| sg219-9 | taatacgactcactataGGGTCTTCAGAATCAGCGACgttttagagctagaa |
| sg196-4 | taatacgactcactataGGGAGGCTTCTCAGAATATTgttttagagctagaa |
| sg196-7 | taatacgactcactataGGGAGCCTATGGAGCCATATgttttagagctagaa |
| Common oligo (reverse primer) | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
