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. 2022 Feb 23;8(8):eabm7950. doi: 10.1126/sciadv.abm7950

Fig. 5. APNs transfect multiplexed T cell subsets with significantly higher transfection efficiency than noncognate cell populations.

Fig. 5.

(A) Schematic of functional biodistribution study at cellular level comparing UV-exchanged PA224/Db APNs with folded PA224/Db APNs. (B) Transfection efficiency of PBS and PA224/Db APNs in the major cell populations of spleen and liver. **P = 0.0010 between PBS and UV-exchanged PA224/Db APNs in monocyte cell population; **P = 0.0025 between PBS and UV-exchanged PA224/Db APNs in Kupffer cells; ****P < 0.0001; two-way ANOVA and Dunnett post-test and correction. All data are means ± SD; n = 4 to 5 biologically independent mice. (C) Dose-dependent transfection in two immunodominant flu-specific T cells in PR8 model. Infected mice were treated with a mixture of folded NP366/Db APNs and PA224/Db APNs. n.s., not significant, where P = 0.0606 between PBS and folded APNs (0.03 mg/kg total mRNA dose) in NP366+ flu-specific T cell population; **P = 0.0015 between PBS and folded APNs (0.03 mg/kg) in PA224+ flu-specific T cell population (PA224+); ****P < 0.0001; two-way ANOVA and Sidak post-test and correction. All data are means ± SD; n = 3 biologically independent mice. (D) Schematic of multiplexed transfection study. (E) Multiplexed transfection study showing the UV-exchanged APN library transfect three virus-specific T cell populations simultaneously. ****P < 0.0001; two-way ANOVA and Sidak post-test and correction. All data are means ± SD; n = 5 biologically independent mice.