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. 2022 Feb 23;8(8):eabm1831. doi: 10.1126/sciadv.abm1831

Fig. 4. Inferred CNA and subclonal analysis reveals enrichment of the stress state during chemotherapy.

Fig. 4.

(A) Inferred clonality tree (left), subclonal stress score (middle), and subclonal enrichment during NACT (right) of a representative patient (EOC3) with progressive disease and short PFI (PFI = 14 days). Only subclones that existed in the treatment-naïve samples are included in the subclonal stress score and subclonal enrichment analysis. The subclonal enrichment is measured by the ratio of the relative abundance of post-NACT cells against the relative abundance of treatment-naïve cells. PARPi, PARP inhibitor. (B) Inferred clonality tree (left), subclonal stress score (middle), and subclonal enrichment during NACT (right) of a representative patient (EOC136) with progressive disease and long PFI (PFI = 520 days). (C) Boxplot showing the enrichment of the stress-highest (red) and stress-lowest (blue) CNA subclones during NACT. Only subclones existing in treatment naïve samples (paired Wilcoxon rank-sum test, P = 0.032) were included. Each dot represents a CNA subclone. (D) Boxplots showing the proliferation score of the stress-highest (left; paired Wilcoxon rank-sum test, P = 0.031) and stress-lowest (right; paired Wilcoxon rank-sum test, P = 0.3) CNA subclones before and after chemotherapy. Each dot represents a CNA subclone.