FIG 1.
Bbvac confers sterilizing immunity against infection with the 3 classical Bordetella spp. C57BL/6J mice were intranasally inoculated with PBS (black) or PBS containing 5 × 105 CFU of B. bronchiseptica (blue), Nobivac (green), or Bbvac (red). Another group was intraperitoneally vaccinated with 1/5 of Adacel (purple). Ninety days later, mice were intranasally challenged with PBS containing 5 × 105 CFU of B. bronchiseptica (A), B. pertussis (B), or B. parapertussis (C). Mice were euthanized 7 days postinoculation, and colonies were enumerated in the respiratory tract. Two-way ANOVA, Dunnett's multiple-comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Error bars represent standard error of the mean. n = 4 per condition.