FIG 7.

Bbvac promotes long-lasting IL-17⁺CD4⁺ T cell memory. C57BL/6J mice were vaccinated with PBS or vaccinated with Bbvac. Fifteen months postvaccination, mice were intranasally challenged with B. bronchiseptica (A and D), B. pertussis (B and E), or B. parapertussis (C and F). Mice were euthanized 7 days postinfection to enumerate and characterize the CD4⁺ T cells in the lungs. Two-way ANOVA, Dunnett's multiple-comparison test. *, P < 0.05. Error bars represent standard error of the mean. n = 4 per strain and condition.