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. Author manuscript; available in PMC: 2022 Feb 23.
Published in final edited form as: Cell Rep. 2022 Feb 1;38(5):110309. doi: 10.1016/j.celrep.2022.110309

Figure 5. VEGF-A–induced blood flow recovery consequent to new angiogenesis and arteriogenesis is dependent on macrophage IL-1β expression in response to acute hindlimb ischemia.

Figure 5.

(A and B) Laser Doppler images of flow in the ischemic (I) and contralateral control (C) hindlimbs of control or myeloid IL-1β-deleted mice (mIL-1β KO) at indicated time points before and after femoral artery ligation along with quantitative analysis (B) (**, p = 0.002; ***, p < 0.0001 between control and mIL-1β KO for each time point by ANOVA; n = 12 mice total, six males and six females).

(C and D) Standard histology stained for hematoxylin and eosin of day 3 ischemic gastrocnemius muscle tissue from control or mIL-1β KO mice along with quantification (D) of number of infiltrating inflammatory cells, indicated by yellow arrows (n = 6 mice total, three males and three females). Bar, 100 microns.

(E–H) Immunofluorescence micrographs of ischemic muscle tissue at day 3 post femoral artery ligation from control or mIL-1β KO mice along with quantitation of DAPI+CD68+ (F), DAPI+CD31+ (G), and DAPI+SMA+ (H) cells (***, p = 0.0003 by t test; n = 6 mice, three males and three females). Bar, 100 microns. Data, mean ± SD.