KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NF-κB p65, monoclonal | Cell Signaling Technology | Cat# 8242; RRID: AB_10859369 |
| STAT3, monoclonal | Cell Signaling Technology | Cat# 9139; RRID: AB_331757 |
| VEGF-A, polyclonal | Sigma | Cat# SAB2502119 |
| Cleaved IL-1 β, mouse-specific monoclonal | Cell Signaling Technology | Cat# 52718; RRID: AB_2799421 |
| IL-1 β (A6), mouse-specific monoclonal | Cell Signaling Technology | Cat# 12242; RRID: AB_2715503 |
| Anti-β-Actin Antibody (C4) | Santa Cruz | Cat# sc-47778; RRID: AB_2714189 |
| APC anti-mouse CD68 Antibody | BioLegend | Cat# 137008; RRID: AB_10575300 |
| Anti-Actin, α-Smooth Muscle - Cy3™ antibody, Mouse monoclon | MilliporeSigma | Cat# C6198; RRID: AB_476856 |
| CD31 (PECAM-1) Monoclonal Antibody (390), FITC, eBioscience™ | Thermo Fisher | Cat# 11-0311-85; RRID: AB_465013 |
| Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 680 | Thermo Fisher | Cat# A21088; RRID: AB_10373119 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 680 | Thermo Fisher | Cat# A10043; RRID: AB_2534018 |
| IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody | Thermo Fisher | Cat# 926–32212; RRID: AB_621847 |
| Biological samples | ||
| Bone marrow-derived macrophages | Davies and Gordon (2005); Healy et al. (2020) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| JSH 23 (NF-κB inhibitor) | Abcam | Cat# ab144824 |
| Stattic (STAT3 inhibitor) | Abcam | Cat# ab120952 |
| Lipopolysaccharides from E. coli 0111:B4 | Sigma Aldrich | Cat# L2630 |
| Recombinant Mouse IFN-γ (carrier-free) | BioLegend | Cat# 575304 |
| Recombinant Mouse IL-13 (carrier-free) | BioLegend | Cat# 575904 |
| Recombinant Mouse IL-4 | BioLegend | Cat# 574304 |
| Cholesterol | MilliporeSigma | Cat# C3045 |
| ProLong™ Gold Antifade Mountant with DAPI | Thermo Fisher | Cat# P36931 |
| Critical commercial assays | ||
| Mouse VEGF DuoSet ELISA | R&D Systems | Cat# DY493; RRID: AB_2884000 |
| ELISA MAX™ Deluxe Set Mouse IL-1 β | BioLegend | Cat# 432604 |
| Nuclear Extraction Kit | Abcam | Cat# ab113474 |
| HIF-1 alpha Transcription Factor Assay Kit | Abcam | Cat# ab133104 |
| STAT3 Transcription Factor Assay Kit (Colorimetric) | Abcam | Cat# ab207229 |
| NFkB p65 Transcription Factor Assay Kit | Abcam | Cat# ab133112 |
| Chromatin Immunoprecipitation (ChIP) Assay Kit | Millipore | Cat# 17–295 |
| Standard Macrophage Depletion Kit (Clodrosome® + Encapsome®) | Fisher Scientific | Cat# NC1361099 |
| Mouse Macrophage Nucleofector™ Kit | Lonza | Cat# VPA-1009 |
| RNeasy Mini Kit | Qiagen | Cat# 74106 |
| Click-iT Nascent RNA Capture Kit | Invitrogen/Life Technologies | Cat# C10365 |
| Deposited data | ||
| Raw and analyzed RNA-Seq data | This manuscript | GEO: GSE191152 |
| Experimental models: Organisms/strains | ||
| Mus musculus: C57BL/6J | The Jackson Laboratory | Cat# 000664; RRID: IMSR_JAX:000664 |
| Mus musculus: Csf1tmlercmmer, FVB-Tg(Csf1r-cre/Esr1*)1Jwp/J | The Jackson Laboratory | Cat# 019098; RRID: IMSR_JAX:019098 |
| Mus musculus: IL-1R−/−, B6.129S7-Il1r1tm1Imx/J | The Jackson Laboratory | Cat# 003245; RRID: IMSR_JAX:003245 |
| Mus musculus: Ai9 (RCL-tdT), B6.Cg-Gt(ROSA) 26Sottm9(CAG-tdTomato)Hze/j | The Jackson Laboratory | Cat# 007909; RRID: IMSR_JAX:007909 |
| Mus musculus: IL-1βfl/fl | The Morrison Laboratory and The Brown University Mouse Transgenic and Gene Targeting Facility | This manuscript. |
| Oligonucleotides | ||
| HPRT, 5’-GACCGGTCCCGTCATGCCGA-3’ (sense) and 5’-TGGCCTCCCATCTCCTCCATGACA-3’ (antisense) | Integrated DNA Technologies | N/A |
| GAPDH, 5’-GTGTGAACGGATTTGGCCG-3’ (sense) and 5’-GTGATGGGCTTCCCGTTGAT-3’ (antisense) | Integrated DNA Technologies | N/A |
| IL-1 β, 5’-AAAGATGAAGGGCTGCTTCC-3’ (sense) and 5’-GTCCACGGGAAAGACACAGG-3’ (antisense) | Integrated DNA Technologies | N/A |
| VEGF-A, 5’-ACTGGACCCTGGCTTTACTGC-3’ (sense) and 5’-TGATCCGCATGATCTGCATGGTG-3’ (antisense) | Integrated DNA Technologies | N/A |
| VEGF-A165a, 5’-CAGAAAATCACTGTGAGCCTTGTT-3’ (sense) and 5’-CTTGGCTTGTCACATCTGCAA-3’ (antisense) | Integrated DNA Technologies | N/A |
| VEGF-A165b, 5’-CAGAAAATCACTGTGAGCCTTGTT-3’ (sense) and 5’-CTTTCCGGTGAGAGGTCTGC-3’ (antisense) | Integrated DNA Technologies | N/A |
| TNF-α, 5’-CACGTCGTAGCAAACCACCAA-3’ (sense) and 5’-AGCAAATCGGCTGACGGTGT-3’ (antisense) | Integrated DNA Technologies | N/A |
| NOS2, 5’-CGGCAAACATGACTTCAGGC-3’ (sense) and 5’-GCACATCAAAGCGGCCATAG-3’ (antisense) | Integrated DNA Technologies | N/A |
| ARG1, 5’-ACATTCGCTTGCGAGACGTA-3’ (sense) and 5’-ATCACCTTGCCAATCCCCAG-3’ (antisense) | Integrated DNA Technologies | N/A |
| FIZZ1, 5’-CCTGCTGGGATGACTGCTAC-3’ (sense) and 5’-CAGTGGTCCAGTCAACGAGT-3’ (antisense) | Integrated DNA Technologies | N/A |
| YM1, 5’-GCTTTTGACGAAGAATCTGTGGAC-3’ (sense) and 5’ TCAGGGATCTTGTACCCAGAC 3’ (antisense) | Integrated DNA Technologies | N/A |
| VEGF-A promoter STAT3 ChIP, 5’-TCCACAGGTCGTCTCACTCC-3’ (sense) and 5’-GCAGTCATCAGGAGCTAGGG-3’ (antisense) | Integrated DNA Technologies | N/A |
| VEGF-A promoter NF-κB ChIP, 5’-TACAGCCTCTGTTATGCCACG-3’ (sense) and 5’-TGGGTTTCCTCACCTGATCC-3’ (antisense) | Integrated DNA Technologies | N/A |
| Recombinant DNA | ||
| pcDNA3.1-(empty)-TAG | Addgene | Cat# 138209; RRID: Addgene_138209 |
| Stat3-C Flag pRc/CMV | Addgene | Cat# 8722; RRID: Addgene_8722 |
| IKK-2 S177E S181E | Addgene | Cat# 11105; RRID: Addgene_11105 |
| Software and algorithms | ||
| Prism 9 Version 9.3.1 (350), December 7, 2021 | GraphPad | https://www.graphpad.com/support/faq/prism-931-release-notes/ |
| Image J Version: 2.0.0-rc-69/1.52p Build:269a0ad53f Date 2018-12-04T11:30:09+000 | Schneider et al. (2012) | https://imagej.net/software/fiji/downloads |
| moorLDI Version 6.1 | Moor Instruments | https://www.moor.co.uk/en-us/products/control/moorldi2-research-software/ |