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. Author manuscript; available in PMC: 2022 Feb 23.
Published in final edited form as: Cell Rep. 2022 Feb 1;38(5):110285. doi: 10.1016/j.celrep.2021.110285

Figure 6. Constitutive HER3 signaling occurs almost entirely from the plasma membrane, not the intracellular pools.

Figure 6.

(A) The surface of CHO cells expressing HER2 and HER3 was biotinylated using a cell-impermeable reagent. The entire surface proteome was then depleted from cell lysates using streptavidin beads, and HER2-HER3 expression and signaling activity was assayed in the intracellular lysate as shown. Lane 3 shows the membrane-depleted intracellular lysate; all other lanes are various negative controls. S indicates depletion by streptavidin beads, D indicates dummy beads, and – indicates no beads.

(B) The same experiment was performed on HER2-amplified HCC1569 breast cancer cells. HER3 signaling in the intracellular lysate was assayed as shown. The streptavidin immunoblot shows the total depletion of the surface proteome in experimental lane 3.