KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Trastuzumab, anti-HER2 | Genentech | Herceptin |
| Pertuzumab, anti-HER2 | Genentech | Perjeta |
| si4D5 anti-HER2 mAb | Absolute Antibody | ab01093-1.32 |
| Mouse IgG | ThermoFisher | 02-6502 |
| Human IgG | ThermoFisher | 31154 |
| Human IgM | ThermoFisher | 31146 |
| Human IgM-biotin-SP | Jackson ImmunoResearch | 009-060-012 |
| anti-HER2 | SantaCruz Biotechnology | C-18 #284 |
| anti-HER3 | SantaCruz Biotechnology | 5A12 #81455 |
| anti-HER3 biotin | SantaCruz Biotechnology | C-17/custom |
| anti-bovine IgG biotin | SantaCruz Biotechnology | 2420 |
| anti-myc | SantaCruz Biotechnology | 9E10 #40 |
| anti-actin | SantaCruz Biotechnology | I-19 #1616 |
| anti-HA | SantaCruz Biotechnology | Y11 sc-805 |
| anti-HA | SantaCruz Biotechnology | F-7 sc-7392 |
| anti-p-HER3 Y1289 | Cell Signaling Technology | 4791 |
| anti-p-HER2 Y1248 | Cell Signaling Technology | 2247 |
| anti-myc | Cell Signaling Technology | 71D0 #2278 |
| anti-streptavidin | Cell Signaling Technology | 3999 |
| anti-Flag | Clontech | 635691 |
| anti-Flag beads | Clontech | 635686 |
| anti-V5 | Invitrogen | R960-25 |
| anti-rabbit IgG-HRP | GE Healthcare | NA9340 |
| anti-mouse IgG-HRP | Cell Signaling Technology | 7076 |
| anti-goat IgG-HRP | SantaCruz Biotechnology | 2020 |
| anti-mouse IgG Alexa 546 | Invitrogen | A11630 |
| Chemicals, peptides, and recombinant proteins | ||
| Lapatinib | GlaxoSmithKline | Tykerb |
| Fetal bovine serum | Gemini Bioproducts | 100-106 |
| Penicillin-Streptomycin-Glutamine | ThermoFisher | 10378016 |
| Heregulin β1 | SigmaAldrich | H0786 |
| Phosphatase inhibitor cocktail | Roche | 04906845001 |
| Lipofectamine 2000 | ThermoFisher | 11668500 |
| JetPrime transfection reagent | Polyplus | 101000015 |
| Transdux reagent | SystemsBio | LV850A-1 |
| Optimem media | ThermoFisher | 31985070 |
| protein G Sepharose 4 fast flow | GE Healthcare | 17-0618-02 |
| streptavidin conjugated agarose | SigmaAldrich | 85881 |
| streptavidin HRP | Cell Signaling Technology | 3999 |
| ProLong Gold antifade reagent | Life Technologies | P36930 |
| Hoechst 33342 | ThermoFisher | 62249 |
| VectaShield anti-fade with DAPI | Vector Laboratories | H-1200 |
| SNAP Cell Oregon Green | New England Biolabs | S9104S |
| SNAP Cell 647-SiR | New England Biolabs | S9102S |
| CLIP Cell TMR Star | New England Biolabs | S9219S |
| CLIP Cell 505 | New England Biolabs | S9217S |
| Paraformaldehyde | Alfa Aesar | 43368 |
| transdux reagent | SystemsBio | LV850A-1 |
| Puromycin | ThermoFisher | A1113802 |
| Critical commercial assays | ||
| Pierce BCA assay | ThermoFisher | 23227 |
| QuikChange II Site-directed mutagenesis kit | Stratagene | 200521 |
| Experimental models: Cell lines | ||
| Hamster: CHO-K1 Chinese hamster ovary cells | ATCC | CCL-61 |
| Human: MCF-7 breast cancer cells | ATCC | HTB-22 |
| Human: SkBr3 breast cancer cells | ATCC | HTB-30 |
| Human: HCC1569 breast cancer cells | ATCC | CRL-2330 |
| Experimental models: Organisms/strains | ||
| NSG (NOD-scid IL2Rgammanull) mice | Jackson Labs | 005557 |
| Recombinant DNA | ||
| pDONR221 | ThermoFisher | 12536017 |
| pENTR4 | ThermoFisher | A10465 |
| pDONR223-HER3 | gift from William Hahn & David Root - Addgene | 23874 |
| pDEST40-2XFL | ThermoFisher / modified | 12274015 |
| pDEST40-2XHA | ThermoFisher / modified | 12274015 |
| pDEST40-nSNAP2XHA | ThermoFisher / modified | 12274015 |
| pDEST40-cSNAP2XFL | ThermoFisher / modified | 12274015 |
| pLEX-IRES-GFP destination | modified from pLEX307, gift of David Root, Addgene | 41392 |
| pLenti-CMV/TO-Hygro-destination | gift of Eric Campeau & Paul Kaufman, Addgene | 17291 |
| Other | ||
| glass slides | ThermoFisher | 12-550-15 |