Figure 6.

CPE-derived EVs containing miR-155-5p suppressed Rheb to aggravate IBI. (a) The expressions of miR-155-5p in mouse brain tissues were determined by RT-qPCR. (b) Rheb mRNA levels in mouse brains were determined by RT-qPCR. (c) The mass ratio of the ipsilateral (right) hemisphere to the contralateral (left) hemisphere of the brain in tMCAO mice was calculated. (d) The neurological function of mice was measured by mNSS. (e) Cerebral infarction in tMCAO mice was detected by TTC. (f) Protein levels of Rheb, mTORC1, and NLRP3 in brain tissues were detected by western blotting. (g, h) Levels of IL-18 (g) and IL-1β (h) in the brain tissue extracts of tMCAO mice were determined by ELISA. (i) Autophagy-associated proteins' expressions in cotreated mouse brain tissues were measured by western blotting. ^∗p < 0.05 vs. sham-operated mice. ^#p < 0.05 vs. oe-NC-infected mice. ^&p < 0.05 vs. mice cotreated with oe-Rheb+EV and agomir-NC (n = 3).