Figure 4.

Expression of genes related to longevity in D. melanogaster supplemented with PTS. Flies were supplemented with 100 μM PTS for 15 days (PTS1) or 60 days (PTS2). Total RNA was isolated, and the mRNA levels of A) Sir2, B) Notch, and C) foxo were quantified using RT-PCR normalized to RPL32 reference gene as reported in Materials and Methods. Triplicate reactions were performed for each experiment. Each bar represents the mean ± SEM of three independent experiments. Data relative to 15 days and 60 days were grouped into one graph but analyzed separately by Student's t-test. ∗p <0.05 with respect to the corresponding controls, CTR1 or CTR2.