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. 2022 Feb 10;13:773209. doi: 10.3389/fnsyn.2021.773209

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Copyright © 2022 Holderith, Aldahabi and Nusser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conditional knock-out of Munc13-2 does not change the expression and distribution of Elfn1 and mGluR7. (A–E) Double immunolabeling for Cre and either Munc13-2 (A) or mGluR1α (B), or Elfn1 (C) or mGluR7 (D) or Munc13-1 (E) in the dorsal CA1 area of the non-injected hemisphere. (F–J) Same as in (A–E), but the images are from the hemisphere that has been injected with AAV expressing Cre-recombinase. Immunolabeled Cre (green) is visible in most CA1 PC nuclei (green). Note the lack of immunolabeling for Munc13-2 in the outer part of the stratum oriens, demonstrating the efficient removal of the protein, while there is no detectable change in the immunolabeling for mGluR1α, Elfn1, mGluR7, and Munc13-1. Maximum intensity projection of 4 confocal images separated by 1 μm. str. pyr, stratum pyramidale; str. oriens, stratum oriens.