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. 2021 Dec 13;15(2):327–337. doi: 10.1038/s41385-021-00472-w

Fig. 3. HVEM and LTβR-mediated mechanisms contribute to fibroblast-eosinophil tethering in co-culture.

Fig. 3

a Representative haematoxylin/eosin stained co-cultures of fibroblasts and eosinophils in fibroblasts transfected with non-targeting control RNA (siNTC) or siRNAs against HVEM (siHVEM) or LTβR (siLTβR) in vehicle or LIGHT treated (24 h) cells followed by co-culture with eosinophils for 8 h in the absence of LIGHT (n = 3). Quantification of total eosinophils (b) or eosinophil clusters (c) in cells treated as in a (each dot represents fibroblasts from an independent donor). *p < 0.05.