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. 2021 Oct 20;15(2):301–313. doi: 10.1038/s41385-021-00462-y

Fig. 7. 4-OI attenuates mitochondrial oxidative metabolism and superoxide generation.

Fig. 7

Metabolic flux (XFp) of a mitochondrial stress test representing (a) OCR and (b) ECAR were graphed over time. Arrowhead indicates drug injection point. HDM and STING stimulated BMDC were incubated with 4-OI (50 µM) for 18 h before the XFp assay. All plots represent one of three experiments. c A bar graph shows the percent change in mean fluorescence intensity of MitoSOX+ CD11b+ BMDC treated with different doses of 4-OI compared to unstimulated control. d qPCR of antioxidant genes and e representative western blot of HDM and STING-stimulated BMDC with or without 4-OI. Data represent the mean ± S.E from one experiment out of three independent repeats (n = 6, *P < 0.05 and **P < 0.01 one-way ANOVA with Sidak’s multiple comparison test).