Skip to main content
. 2021 Dec 20;15(2):257–267. doi: 10.1038/s41385-021-00473-9

Fig. 3. Skin-homing receptors are up-regulated on mesLN and circulating CD4+ T cells after H. polygyrus infection.

Fig. 3

an Mice were infected with H. polygyrus (Hp) or not (−). ah Mesenteric lymph nodes (mesLNs) collected two weeks after infection. a Representative FACS plots of CCR4 staining gated on mesLN CD4+ T cells. b, c Frequencies and absolute numbers of CCR4+ cells out of CD4+ T cells. d Representative FACS plots of CCR10 staining gated on mesLN CD4+ T cells. e, f Frequencies and absolute numbers of CCR10+ cells out of CD4+ T cells. g, h Fold change of Ccr4 and Ccr10 mRNA expression in CD4+ T cells bead isolated from mesLNs. ip Blood collected at indicated time points after infection. i Representative FACS plots of CCR4 staining gated on blood CD4+ T cells. j, k Frequencies of CCR4+ cells out of CD4+ T cells. l Representative FACS plots of CCR10 staining gated on blood CD4+ T cells. m, n Frequencies of CCR10+ cells out of CD4+ T cells. o, p Mice were treated for 7 days with 2% dextran sodium sulphate (DSS) in the drinking water and rested for 7 days. Frequencies of CCR4+ and CCR10+ cells out of CD4+ T cells, respectively. Samples were analysed by flow cytometry (af, ip) or qPCR (gh). In af, ip, one out of at least two independent experiments with similar results are shown. g, h shows pooled data from two independent experiments. Each dot represents one mouse (n ≥ 4 per group) and bars indicate the mean ± SEM. Statistical differences were analysed by unpaired t-tests (c, e, h, m) or Mann–Whitney U test (b, f, g, j, k, n) and depicted as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

HHS Vulnerability Disclosure